PrimePCR™ SYBR® Green Assay: EIF2S3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028305

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012108
Assay Design:   Intron-spanning
Chromosome Location:   X:24073070-24073759question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000253039 ENST00000423068

Gene Information

The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit. [provided by RefSeq Jan 2010]

Gene Symbol:   EIF2S3
Gene Name:   eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
Aliases:   EIF2, EIF2G, EIF2gamma, eIF-2gA
RefSeq:   NC_000023.10 NG_016387.1 NT_167197.1
Ensembl:   ENSG00000130741
Entrez:   1968
UniGene:   Hs.539684
Chromosome Mapping:   Xp22.2-p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 33.630000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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