PrimePCR™ SYBR® Green Assay: CHM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012100
Assay Design:   Intron-spanning
Chromosome Location:   X:85233852-85282542question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000357749 ENST00000358786

Gene Information

This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia; also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid retinal pigment epithelium and retina. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq Feb 2009]

Gene Symbol:   CHM
Gene Name:   choroideremia (Rab escort protein 1)
Aliases:   DXS540, FLJ38564, GGTA, HSD-32, MGC102710, REP-1, TCD
RefSeq:   NC_000023.10 NG_009874.1 NT_011651.17
Ensembl:   ENSG00000188419
Entrez:   1121
UniGene:   Hs.496449
Chromosome Mapping:   Xq21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.680000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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