PrimePCR™ SYBR® Green Assay: CCDC120, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012053
Assay Design:   Intron-spanning
Chromosome Location:   X:48916525-48919527question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000376396 ENST00000422185 ENST00000536628

Gene Information

This gene encodes a protein that contains a coiled-coil domain. Four alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq Nov 2009]

Gene Symbol:   CCDC120
Gene Name:   coiled-coil domain containing 120
Aliases:   Not Available
RefSeq:   NC_000023.10 NT_079573.4 NG_016255.1
Ensembl:   ENSG00000147144
Entrez:   90060
UniGene:   Hs.522643
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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