PrimePCR™ SYBR® Green Assay: STYK1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028269

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012019
Assay Design:   Intron-spanning
Chromosome Location:   12:10787166-10789727question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000075503 ENST00000538867 ENST00000535345

Gene Information

Receptor protein tyrosine kinases like STYK1 play important roles in diverse cellular and developmental processes such as cell proliferation differentiation and survival (Liu et al. 2004 [PubMed 15150103]).[supplied by OMIM Mar 2008]

Gene Symbol:   STYK1
Gene Name:   serine/threonine/tyrosine kinase 1
Aliases:   DKFZp761P1010, NOK, SuRTK106
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000060140
Entrez:   55359
UniGene:   Hs.24979
Chromosome Mapping:   12p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.620000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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