PrimePCR™ SYBR® Green Assay: PRRC2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028257

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011995
Assay Design:   Intron-spanning
Chromosome Location:   9:134353927-134354736question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000405995 ENST00000451855 ENST00000357304 ENST00000458550 ENST00000418650 ENST00000372249

Gene Information

Description Not Available

Gene Symbol:   PRRC2B
Gene Name:   proline-rich coiled-coil 2B
Aliases:   BAT2L, BAT2L1, DKFZp781F05101, DKFZp781K12107, KIAA0515, LQFBS-1, MGC10526
RefSeq:   NC_000009.11 NG_008896.1 NT_035014.4
Ensembl:   ENSG00000130723
Entrez:   84726
UniGene:   Hs.495349
Chromosome Mapping:   9q34.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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