PrimePCR™ SYBR® Green Assay: MAP3K13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011976
Assay Design:   Intron-spanning
Chromosome Location:   3:185169123-185181447question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000446828 ENST00000424227 ENST00000443863 ENST00000265026 ENST00000420577 ENST00000535426

Gene Information

The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK MAP2K7/MKK7 which suggests a role in the JNK signaling pathway. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP3K13
Gene Name:   mitogen-activated protein kinase kinase kinase 13
Aliases:   LZK, MEKK13, MGC133196
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000073803
Entrez:   9175
UniGene:   Hs.591306
Chromosome Mapping:   3q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 34.940000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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