PrimePCR™ SYBR® Green Assay: TAPT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028250

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011975
Assay Design:   Intron-spanning
Chromosome Location:   4:16189881-16193052question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000405303 ENST00000542770 ENST00000399920 ENST00000304584

Gene Information

This gene encodes a highly conserved putative transmembrane protein. A mutation in the mouse ortholog of this gene results in homeotic posterior-to-anterior transformations of the axial skeleton which are similar to the phenotype of mouse homeobox C8 gene mutants. This gene is proposed to function downstream of homeobox C8 to transduce extracellular patterning information during axial skeleton development. An alternatively spliced transcript variant encoding a substantially different isoform has been described but its biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TAPT1
Gene Name:   transmembrane anterior posterior transformation 1
Aliases:   CMVFR, FLJ90013
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000169762
Entrez:   202018
UniGene:   Hs.479223
Chromosome Mapping:   4p15.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download