PrimePCR™ SYBR® Green Assay: TRMU, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011948
Assay Design:   Intron-spanning
Chromosome Location:   22:46746300-46748180question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000290846 ENST00000381019 ENST00000424260

Gene Information

This gene is a member of the trmU family. It encodes a mitochondria-specific tRNA-modifying enzyme that is required for the 2-thio modification of 5-taurinomethyl-2-thiouridine tRNA-Lys on the wobble position of the anticodon. [provided by RefSeq Jul 2008]

Gene Symbol:   TRMU
Gene Name:   tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Aliases:   MGC99627, MTO2, MTU1, TRMT, TRMT1, TRNT1
RefSeq:   NC_000022.10 NG_012173.1 NT_011520.12
Ensembl:   ENSG00000100416
Entrez:   55687
UniGene:   Not Available
Chromosome Mapping:   22q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.850000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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