This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028229
PrimePCR™ PreAmp for SYBR® Green Assay: ITPKA, Human
PrimePCR™ Template for SYBR® Green Assay: ITPKA, Human
Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 145-trisphosphate to Ins(1345)P4. The activity of the inositol 145-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase calcium/calmodulin- dependent protein kinase II and protein kinase C in vitro.[provided by RefSeq Apr 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.