This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028223
PrimePCR™ PreAmp for SYBR® Green Assay: ZMYND19, Human
PrimePCR™ Template for SYBR® Green Assay: ZMYND19, Human
ZMYND19 is a MYND zinc finger domain-containing protein that binds to the C terminus of melanin-concentrating hormone receptor-1 (MCHR1; MIM 601751) (Bachner et al. 2002 [PubMed 12208518]) and to the N termini of alpha-tubulin (TUBA1; MIM 191110) and beta-tubulin (TUBB; MIM 191130) (Francke et al. 2005 [PubMed 16039987]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.