PrimePCR™ SYBR® Green Assay: MORN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011852
Assay Design:   Intron-spanning
Chromosome Location:   1:2317267-2318931question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000378531 ENST00000378529 ENST00000449373 ENST00000378527 ENST00000378525

Gene Information

Description Not Available

Gene Symbol:   MORN1
Gene Name:   MORN repeat containing 1
Aliases:   FLJ13941
RefSeq:   NG_016128.1 NC_000001.10 NT_004350.19
Ensembl:   ENSG00000116151
Entrez:   79906
UniGene:   Hs.642701
Chromosome Mapping:   1p36.33-p36.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 33.350000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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