PrimePCR™ SYBR® Green Assay: BAIAP2L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011802
Assay Design:   Intron-spanning
Chromosome Location:   7:97939824-97941455question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000005260

Gene Information

This gene encodes a member of the IMD (IRSp53/MIM homology domain) family. Members of this family can be subdivided in two groups the IRSp53-like and MIM-like based on the presence or absence of the SH3 (Src homology 3) domain. The protein encoded by this gene contains a conserved IMD also known as F-actin bundling domain at the N-terminus and a canonical SH3 domain near the C-terminus so it belongs to the IRSp53-like group. This protein is the substrate for insulin receptor tyrosine kinase and binds to the small GTPase Rac. It is involved in signal transduction pathways that link deformation of the plasma membrane and remodeling of the actin cytoskeleton. It also promotes actin assembly and membrane protrusions when overexpressed in mammalian cells and is essential to the formation of a potent actin assembly complex during EHEC (Enterohemorrhagic Escherichia coli) pedestal formation. [provided by RefSeq Oct 2009]

Gene Symbol:   BAIAP2L1
Gene Name:   BAI1-associated protein 2-like 1
RefSeq:   NM_018842
Ensembl:   ENSG00000006453
Entrez:   55971
UniGene:   Hs.656063
Chromosome Mapping:   chr7:97920961-98030427

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.020000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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