PrimePCR™ SYBR® Green Assay: AHNAK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011787
Assay Design:   Intron-spanning
Chromosome Location:   11:62302586-62303468question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000530124 ENST00000257247 ENST00000533365 ENST00000378024 ENST00000533743 ENST00000530285 ENST00000528508 ENST00000531324

Gene Information

Description Not Available

Gene Symbol:   AHNAK
Gene Name:   AHNAK nucleoprotein
Aliases:   AHNAKRS, MGC5395
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000124942
Entrez:   79026
UniGene:   Hs.502756
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995700
y-intercept 34.290000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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