PrimePCR™ SYBR® Green Assay: RSRC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011695
Assay Design:   Intron-spanning
Chromosome Location:   3:157840054-157920873question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000480820 ENST00000494002 ENST00000295930 ENST00000471994 ENST00000464171 ENST00000312179 ENST00000475278 ENST00000476899

Gene Information

Serine- and arginine-rich (SR) proteins and SR-related proteins like RSRC1 function in spliceosome assembly and participate in multiple steps of mRNA splicing (Cazalla et al. 2005 [PubMed 15798186]).[supplied by OMIM Apr 2010]

Gene Symbol:   RSRC1
Gene Name:   arginine/serine-rich coiled-coil 1
Aliases:   MGC12197, SFRS21, SRrp53
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000174891
Entrez:   51319
UniGene:   Hs.591283
Chromosome Mapping:   3q25.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.460000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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