PrimePCR™ SYBR® Green Assay: RSPO4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028102

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011607
Assay Design:   Intron-spanning
Chromosome Location:   20:947925-948734question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000217260 ENST00000400634

Gene Information

This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide cysteine-rich/furin-like domain thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq Sep 2009]

Gene Symbol:   RSPO4
Gene Name:   R-spondin family, member 4
Aliases:   C20orf182, CRISTIN4, FLJ16018
RefSeq:   NC_000020.10 NG_013043.1 NT_011387.8
Ensembl:   ENSG00000101282
Entrez:   343637
UniGene:   Hs.444980
Chromosome Mapping:   20p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.580000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download