PrimePCR™ SYBR® Green Assay: NAPSA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011603
Assay Design:   Intron-spanning
Chromosome Location:   19:50863072-50864296question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000253719

Gene Information

The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments or pro-parts are deemed important for correct folding targeting and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [provided by RefSeq Jul 2008]

Gene Symbol:   NAPSA
Gene Name:   napsin A aspartic peptidase
Aliases:   KAP, Kdap, NAP1, NAPA, SNAPA
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000131400
Entrez:   9476
UniGene:   Hs.512843
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 36.250000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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