This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028097
PrimePCR™ PreAmp for SYBR® Green Assay: T, Human
PrimePCR™ Template for SYBR® Green Assay: T, Human
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element the palindromic T-site. It binds through a region in its N-terminus called the T-box and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.