PrimePCR™ SYBR® Green Assay: GBP6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028029

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011460
Assay Design:   Intron-spanning
Chromosome Location:   1:89849765-89850851question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000370456 ENST00000544311 ENST00000535065

Gene Information

Guanylate-binding proteins such as GBP6 are induced by interferon and hydrolyze GTP to both GDP and GMP (Olszewski et al. 2006 [PubMed 16689661]).[supplied by OMIM Dec 2008]

Gene Symbol:   GBP6
Gene Name:   guanylate binding protein family, member 6
Aliases:   DKFZp686G0786, FLJ39135
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000183347
Entrez:   163351
UniGene:   Hs.254338
Chromosome Mapping:   1p22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 33.570000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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