PrimePCR™ SYBR® Green Assay: TSNAX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028023

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011451
Assay Design:   Intron-spanning
Chromosome Location:   1:231678227-231696921question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000366639 ENST00000413309

Gene Information

This gene encodes a protein which specifically interacts with translin a DNA-binding protein that binds consensus sequences at breakpoint junctions of chromosomal translocations. The encoded protein contains bipartite nuclear targeting sequences that may provide nuclear transport for translin which lacks any nuclear targeting motifs. [provided by RefSeq Jul 2008]

Gene Symbol:   TSNAX
Gene Name:   translin-associated factor X
Aliases:   DISC1, TRAX
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000116918
Entrez:   7257
UniGene:   Not Available
Chromosome Mapping:   1q42.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.530000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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