PrimePCR™ SYBR® Green Assay: DOC2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011444
Assay Design:   Intron-spanning
Chromosome Location:   17:11295-11975question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000496357 ENST00000343572

Gene Information

There are at least two protein isoforms of the Double C2 protein namely alpha (DOC2A) and beta (DOC2B) which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2B is expressed ubiquitously and is suggested to be involved in Ca(2+)-dependent intracellular vesicle trafficking in various types of cells. [provided by RefSeq Jul 2008]

Gene Symbol:   DOC2B
Gene Name:   double C2-like domains, beta
Aliases:   DOC2BL
RefSeq:   NC_000017.10 NG_021143.1 NT_024972.8 NW_003315952.1
Ensembl:   ENSG00000187939
Entrez:   8447
UniGene:   Hs.551705
Chromosome Mapping:   17p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download