PrimePCR™ SYBR® Green Assay: DMBT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028016

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DMBT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DMBT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011434
Assay Design:   Intron-spanning
Chromosome Location:   10:124396732-124399604question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000338354 ENST00000368915 ENST00000341278 ENST00000368953 ENST00000339871 ENST00000368942 ENST00000327438 ENST00000344338 ENST00000339712 ENST00000330163 ENST00000368909 ENST00000368955 ENST00000368956 ENST00000359586 ENST00000344467

Gene Information

Loss of sequences from human chromosome 10q has been associated with the progression of human cancers. The gene DMBT1 was originally isolated based on its deletion in a medulloblastoma cell line. DMBT1 is expressed with transcripts of 6.0 7.5 and 8.0 kb in fetal lung and with one transcript of 8.0 kb in adult lung although the 7.5 kb transcript has not been characterized. The DMBT1 protein is a glycoprotein containing multiple scavenger receptor cysteine-rich (SRCR) domains separated by SRCR-interspersed domains (SID). Transcript variant 2 (8.0 kb) has been shown to bind surfactant protein D independently of carbohydrate recognition. This indicates that DMBT1 may not be a classical tumor supressor gene but rather play a role in the interaction of tumor cells and the immune system. [provided by RefSeq Jul 2008]

Gene Symbol:   DMBT1
Gene Name:   deleted in malignant brain tumors 1
Aliases:   FLJ61058, GP340, MGC164738, muclin
RefSeq:   NC_000010.10 NT_030059.13 NG_012644.1
Ensembl:   ENSG00000187908
Entrez:   1755
Chromosome Mapping:   10q26.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.340000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download