This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027999
PrimePCR™ PreAmp for SYBR® Green Assay: TNNC2, Human
PrimePCR™ Template for SYBR® Green Assay: TNNC2, Human
Troponin (Tn) a key protein complex in the regulation of striated muscle contraction is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase the Tn-T subunit binds tropomyosin and Tn-C while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.