PrimePCR™ SYBR® Green Assay: RNF150, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011370
Assay Design:   Intron-spanning
Chromosome Location:   4:141868810-141870505question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000379512 ENST00000506101 ENST00000515673 ENST00000507500 ENST00000420921 ENST00000306799

Gene Information

Description Not Available

Gene Symbol:   RNF150
Gene Name:   ring finger protein 150
Aliases:   MGC125502
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000170153
Entrez:   57484
UniGene:   Hs.659104
Chromosome Mapping:   4q31.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.480000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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