PrimePCR™ SYBR® Green Assay: NDRG4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027949

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011287
Assay Design:   Intron-spanning
Chromosome Location:   16:58540841-58541871question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000258187 ENST00000394282 ENST00000394279 ENST00000356752 ENST00000421602

Gene Information

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq Jun 2011]

Gene Symbol:   NDRG4
Gene Name:   NDRG family member 4
Aliases:   DKFZp686I1615, FLJ30586, FLJ42011, KIAA1180, MGC19632, SMAP-8
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000103034
Entrez:   65009
UniGene:   Hs.322430
Chromosome Mapping:   16q21-q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.730000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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