PrimePCR™ SYBR® Green Assay: PROS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011284
Assay Design:   Intron-spanning
Chromosome Location:   3:93629477-93646164question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000394236 ENST00000407433 ENST00000348974 ENST00000472684

Gene Information

This gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. [provided by RefSeq Feb 2009]

Gene Symbol:   PROS1
Gene Name:   protein S (alpha)
Aliases:   PROS, PS21, PS22, PS23, PS24, PS25, PSA
RefSeq:   NC_000003.11 NG_009813.1 NT_005612.16
Ensembl:   ENSG00000184500
Entrez:   5627
UniGene:   Hs.64016
Chromosome Mapping:   3q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.360000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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