PrimePCR™ SYBR® Green Assay: UQCRFS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011258
Assay Design:   Intron-spanning
Chromosome Location:   19:29698996-29703921question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000304863

Gene Information

Description Not Available

Gene Symbol:   UQCRFS1
Gene Name:   ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
Aliases:   RIP1, RIS1, RISP, UQCR5
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000169021
Entrez:   7386
UniGene:   Hs.170107
Chromosome Mapping:   19q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.640000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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