This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027935
PrimePCR™ PreAmp for SYBR® Green Assay: AHSG, Human
PrimePCR™ Template for SYBR® Green Assay: AHSG, Human
Alpha2-HS glycoprotein (AHSG) a glycoprotein present in the serum is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions such as endocytosis brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix and it has therefore been postulated that it participates in the development of the tissues. However its exact significance is still obscure. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.