PrimePCR™ SYBR® Green Assay: FRS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027932

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011246
Assay Design:   Intron-spanning
Chromosome Location:   12:69955990-69962841question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000547219 ENST00000549921 ENST00000550316 ENST00000548154 ENST00000550389 ENST00000550937 ENST00000549092 ENST00000550169 ENST00000551325 ENST00000299293 ENST00000397997

Gene Information

Description Not Available

Gene Symbol:   FRS2
Gene Name:   fibroblast growth factor receptor substrate 2
Aliases:   FRS2A, FRS2alpha, SNT, SNT-1, SNT1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000166225
Entrez:   10818
UniGene:   Hs.593446
Chromosome Mapping:   12q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.010000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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