PrimePCR™ SYBR® Green Assay: PMVK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027910

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011204
Assay Design:   Intron-spanning
Chromosome Location:   1:154904853-154909132question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000368467

Gene Information

This gene encodes a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of this protein is regulated by sterol and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase the rate-limiting enzyme of cholesterol biosynthesis. [provided by RefSeq Sep 2011]

Gene Symbol:   PMVK
Gene Name:   phosphomevalonate kinase
Aliases:   HUMPMKI, PMK, PMKA, PMKASE
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163344
Entrez:   10654
UniGene:   Hs.30954
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.400000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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