PrimePCR™ SYBR® Green Assay: SMPDL3B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027884

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SMPDL3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SMPDL3B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011145
Assay Design:   Intron-spanning
Chromosome Location:   1:28271897-28279739question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000373894 ENST00000411604 ENST00000373888 ENST00000549094 ENST00000412515 ENST00000373890

Gene Information

Description Not Available

Gene Symbol:   SMPDL3B
Gene Name:   sphingomyelin phosphodiesterase, acid-like 3B
Aliases:   ASML3B
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000130768
Entrez:   27293
Chromosome Mapping:   1p35.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.410000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download