PrimePCR™ SYBR® Green Assay: CINP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011141
Assay Design:   Intron-spanning
Chromosome Location:   14:102822176-102825862question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000216756 ENST00000536961 ENST00000541568

Gene Information

The protein encoded by this gene interacts with CDK2 MCM5 and CDC7 and is associated with the origin recognition complex protein ORC2. It acts as a homodimer and is involved in replication and ATR-mediated checkpoint signaling. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2010]

Gene Symbol:   CINP
Gene Name:   cyclin-dependent kinase 2 interacting protein
Aliases:   MGC849
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100865
Entrez:   51550
UniGene:   Hs.129634
Chromosome Mapping:   14q32.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.350000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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