PrimePCR™ SYBR® Green Assay: KIAA1737, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011091
Assay Design:   Intron-spanning
Chromosome Location:   14:77576293-77579863question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000361786 ENST00000555611 ENST00000554658 ENST00000557115 ENST00000554447 ENST00000555200

Gene Information

Description Not Available

Gene Symbol:   KIAA1737
Gene Name:   KIAA1737
Aliases:   Not Available
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000198894
Entrez:   85457
UniGene:   Hs.709066
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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