PrimePCR™ SYBR® Green Assay: SASH3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027850

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011078
Assay Design:   Intron-spanning
Chromosome Location:   X:128925008-128926348question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000356892 ENST00000443760

Gene Information

The protein encoded by this gene contains a Src homology-3 (SH3) domain and a sterile alpha motif (SAM) both of which are found in proteins involved in cell signaling. This protein may function as a signaling adapter protein in lymphocytes.[provided by RefSeq Sep 2009]

Gene Symbol:   SASH3
Gene Name:   SAM and SH3 domain containing 3
Aliases:   753P9, CXorf9, HACS2, SH3D6C, SLY
RefSeq:   NC_000023.10 NG_013260.1 NT_011786.16
Ensembl:   ENSG00000122122
Entrez:   54440
UniGene:   Hs.61469
Chromosome Mapping:   Xq26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 33.530000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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