PrimePCR™ SYBR® Green Assay: SLC24A6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027840

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC24A6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC24A6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011055
Assay Design:   Intron-spanning
Chromosome Location:   12:113759111-113770622question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000552014 ENST00000546737 ENST00000549181 ENST00000548186 ENST00000549372 ENST00000202831 ENST00000377458

Gene Information

SLC24A6 belongs to a family of potassium-dependent sodium/calcium exchangers that maintain cellular calcium homeostasis through the electrogenic countertransport of 4 sodium ions for 1 calcium ion and 1 potassium ion (Cai and Lytton 2004 [PubMed 14625281]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC24A6
Gene Name:   solute carrier family 24 (sodium/potassium/calcium exchanger), member 6
Aliases:   FLJ22233, NCKX6, NCLX
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000089060
Entrez:   80024
Chromosome Mapping:   12q24.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.110000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download