PrimePCR™ SYBR® Green Assay: RBM19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027839

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011052
Assay Design:   Intron-spanning
Chromosome Location:   12:114395770-114397107question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000545145 ENST00000392561 ENST00000261741

Gene Information

This gene encodes a nucleolar protein that contains six RNA-binding motifs. The encoded protein may be involved in regulating ribosome biogenesis. Multiple alternatively spliced variants encoding the same protein have been identified.[provided by RefSeq Apr 2009]

Gene Symbol:   RBM19
Gene Name:   RNA binding motif protein 19
Aliases:   DKFZp586F1023, KIAA0682
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000122965
Entrez:   9904
UniGene:   Hs.7482
Chromosome Mapping:   12q24.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.410000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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