PrimePCR™ SYBR® Green Assay: ABTB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011033
Assay Design:   Intron-spanning
Chromosome Location:   3:127396665-127398963question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000232744 ENST00000453791 ENST00000468137 ENST00000361019 ENST00000393363

Gene Information

This gene encodes a protein with an ankyrin repeat region and two BTB/POZ domains which are thought to be involved in protein-protein interactions. Expression of this gene is activated by the phosphatase and tensin homolog a tumor suppressor. Alternate splicing results in three transcript variants. [provided by RefSeq Mar 2010]

Gene Symbol:   ABTB1
Gene Name:   ankyrin repeat and BTB (POZ) domain containing 1
Aliases:   BPOZ, BTB3, EF1ABP, MGC20585
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114626
Entrez:   80325
UniGene:   Hs.107812
Chromosome Mapping:   3q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.210000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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