PrimePCR™ SYBR® Green Assay: PPARA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027813

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPARA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPARA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011001
Assay Design:   Intron-spanning
Chromosome Location:   22:46594443-46611188question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000420804 ENST00000407236 ENST00000402126 ENST00000396000 ENST00000262735 ENST00000434345 ENST00000535613

Gene Information

Peroxisome proliferators include hypolipidemic drugs herbicides leukotriene antagonists and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors called PPARs which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha beta/delta and gamma) have been identified. This gene encodes the subtype PPAR-alpha which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene although the full-length nature of only two has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   PPARA
Gene Name:   peroxisome proliferator-activated receptor alpha
Aliases:   MGC2237, MGC2452, NR1C1, PPAR, PPARalpha, hPPAR
RefSeq:   NC_000022.10 NT_011520.12 NG_012204.1
Ensembl:   ENSG00000186951
Entrez:   5465
Chromosome Mapping:   22q12-q13.1,22q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.150000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download