This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027813
PrimePCR™ PreAmp for SYBR® Green Assay: PPARA, Human
PrimePCR™ Template for SYBR® Green Assay: PPARA, Human
Peroxisome proliferators include hypolipidemic drugs herbicides leukotriene antagonists and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors called PPARs which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha beta/delta and gamma) have been identified. This gene encodes the subtype PPAR-alpha which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene although the full-length nature of only two has been determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.