PrimePCR™ SYBR® Green Assay: MLLT3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027809

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010989
Assay Design:   Intron-spanning
Chromosome Location:   9:20360808-20363565question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000380338 ENST00000380323 ENST00000380321 ENST00000429426 ENST00000540751 ENST00000355930

Gene Information

Description Not Available

Gene Symbol:   MLLT3
Gene Name:   myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
Aliases:   AF9, FLJ2035, YEATS3
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000171843
Entrez:   4300
UniGene:   Hs.591085
Chromosome Mapping:   9p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 34.700000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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