PrimePCR™ SYBR® Green Assay: SCMH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027802

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010959
Assay Design:   Intron-spanning
Chromosome Location:   1:41503127-41512141question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000372597 ENST00000372596 ENST00000337495 ENST00000372595 ENST00000326197 ENST00000361705 ENST00000456518 ENST00000402904 ENST00000397174 ENST00000397171 ENST00000361191

Gene Information

Description Not Available

Gene Symbol:   SCMH1
Gene Name:   sex comb on midleg homolog 1 (Drosophila)
Aliases:   Scml3
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000010803
Entrez:   22955
UniGene:   Hs.571874
Chromosome Mapping:   1p34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.930000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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