PrimePCR™ SYBR® Green Assay: C8orf40, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010953
Assay Design:   Intron-spanning
Chromosome Location:   8:42401686-42403841question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000438528 ENST00000417410 ENST00000414154 ENST00000416469 ENST00000490331

Gene Information

Description Not Available

Gene Symbol:   C8orf40
Gene Name:   chromosome 8 open reading frame 40
Aliases:   Not Available
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000176209
Entrez:   114926
UniGene:   Hs.655320
Chromosome Mapping:   8p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 33.680000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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