PrimePCR™ SYBR® Green Assay: MDFI, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027792

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MDFI, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MDFI, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010933
Assay Design:   Intron-spanning
Chromosome Location:   6:41617520-41621127question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000432027 ENST00000419164 ENST00000373051 ENST00000230321 ENST00000373050 ENST00000435476 ENST00000543326

Gene Information

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog I-mf show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq Jul 2008]

Gene Symbol:   MDFI
Gene Name:   MyoD family inhibitor
Aliases:   I-MF, I-mfa
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112559
Entrez:   4188
Chromosome Mapping:   6p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.900000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download