PrimePCR™ SYBR® Green Assay: CARS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010925
Assay Design:   Intron-spanning
Chromosome Location:   11:3038425-3039143question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000380525 ENST00000397111 ENST00000278224 ENST00000397114 ENST00000401769

Gene Information

This gene encodes a class 1 aminoacyl-tRNA synthetase cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5 an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocortical carcinoma and lung ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq Aug 2010]

Gene Symbol:   CARS
Gene Name:   cysteinyl-tRNA synthetase
Aliases:   CARS1, CYSRS, MGC:11246
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000110619
Entrez:   833
UniGene:   Hs.274873
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.160000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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