PrimePCR™ SYBR® Green Assay: ARHGAP21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027786

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARHGAP21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARHGAP21, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010914
Assay Design:   Intron-spanning
Chromosome Location:   10:24918925-24923978question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000396432 ENST00000376410 ENST00000446003 ENST00000416305 ENST00000447364 ENST00000445703 ENST00000535396

Gene Information

ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3 complex (MIM 604221) and F-actin dynamics at the Golgi through control of CDC42 activity (Dubois et al. 2005 [PubMed 15793564]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARHGAP21
Gene Name:   Rho GTPase activating protein 21
Aliases:   ARHGAP10, DKFZp761L0424, FLJ33323, FLJ90108
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000107863
Entrez:   57584
Chromosome Mapping:   10p12.1,10p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.360000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download