PrimePCR™ SYBR® Green Assay: CALB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010913
Assay Design:   Intron-spanning
Chromosome Location:   8:91078126-91081389question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000265431 ENST00000518457 ENST00000523716 ENST00000520613

Gene Information

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq Jul 2008]

Gene Symbol:   CALB1
Gene Name:   calbindin 1, 28kDa
Aliases:   CALB
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000104327
Entrez:   793
UniGene:   Hs.65425
Chromosome Mapping:   8q21.3-q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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