PrimePCR™ SYBR® Green Assay: DCX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010869
Assay Design:   Intron-spanning
Chromosome Location:   X:110644488-110653305question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000338081 ENST00000358070 ENST00000356220 ENST00000488120 ENST00000356915 ENST00000371993

Gene Information

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains which bind microtubules. In the developing cortex cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition the encoded protein interacts with LIS1 the regulatory gamma subunit of platelet activating factor acetylhydrolase and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex leading to epilepsy mental retardation subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2010]

Gene Symbol:   DCX
Gene Name:   doublecortin
Aliases:   DBCN, DC, FLJ51296, LISX, SCLH, XLIS
RefSeq:   NC_000023.10 NT_011651.17 NG_011750.1
Ensembl:   ENSG00000077279
Entrez:   1641
UniGene:   Hs.34780
Chromosome Mapping:   Xq22.3-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 33.090000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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