PrimePCR™ SYBR® Green Assay: HUWE1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027756

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HUWE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HUWE1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010856
Assay Design:   Intron-spanning
Chromosome Location:   X:53619468-53620362question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000342160 ENST00000427052 ENST00000218328 ENST00000262854 ENST00000396323

Gene Information

This gene encodes a member of the HECT E3 ubiquitin ligase family. The HECT domain lies in the C-terminus and contains the active-site cysteine which forms an intermediate ubiquitin-thioester bond. E3 family members are divided into three subfamilies based on their protein-protein interaction domains; this gene encodes a member of the SI(ngle)-HECT E3 subfamily. In lung breast and colorectal carcinomas this gene is highly expressed. Mutations in this gene has been found in 3 unrelated families with X-linked syndromic mental retardation Turner type. [provided by RefSeq Nov 2009]

Gene Symbol:   HUWE1
Gene Name:   HECT, UBA and WWE domain containing 1
Aliases:   ARF-BP1, HECTH9, HSPC272, Ib772, KIAA0312, LASU1, MULE, UREB1
RefSeq:   NC_000023.10 NG_016261.1 NT_011630.14
Ensembl:   ENSG00000086758
Entrez:   10075
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.000000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download