PrimePCR™ SYBR® Green Assay: APOBEC3G, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010825
Assay Design:   Intron-spanning
Chromosome Location:   22:39477500-39479825question
Amplicon Length:   151
Splice Variants Targeted:   ENST00000407997 ENST00000452957

Gene Information

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster thought to result from gene duplication on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq Jul 2008]

Gene Symbol:   APOBEC3G
Gene Name:   apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
Aliases:   ARCD, ARP-9, ARP9, CEM-15, CEM15, FLJ12740, bK150C2.7, dJ494G10.1
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000239713
Entrez:   60489
UniGene:   Hs.660143
Chromosome Mapping:   22q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.270000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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