PrimePCR™ SYBR® Green Assay: EIF2B5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027738

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010804
Assay Design:   Intron-spanning
Chromosome Location:   3:183858475-183859818question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000273783 ENST00000444495 ENST00000544027

Gene Information

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B) a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq Nov 2009]

Gene Symbol:   EIF2B5
Gene Name:   eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Aliases:   CACH, CLE, EIF-2B, EIF2Bepsilon, LVWM
RefSeq:   NC_000003.11 NG_015826.1 NT_005612.16
Ensembl:   ENSG00000145191
Entrez:   8893
UniGene:   Hs.283551
Chromosome Mapping:   3q27.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.270000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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