PrimePCR™ SYBR® Green Assay: UBXN4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010783
Assay Design:   Intron-spanning
Chromosome Location:   2:136505837-136511767question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000272638 ENST00000415164 ENST00000430594

Gene Information

UBXD2 is an integral membrane protein of the endoplasmic reticulum (ER) that binds valosin-containing protein (VCP; MIM 601023) and promotes ER-associated protein degradation (ERAD) (Liang et al. 2006 [PubMed 16968747]).[supplied by OMIM Mar 2008]

Gene Symbol:   UBXN4
Gene Name:   UBX domain protein 4
Aliases:   FLJ23318, KIAA0242, KIAA2042, UBXD2, UBXDC1, erasin
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000144224
Entrez:   23190
UniGene:   Hs.591242
Chromosome Mapping:   2q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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