This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027721
PrimePCR™ PreAmp for SYBR® Green Assay: NCOA2, Human
PrimePCR™ Template for SYBR® Green Assay: NCOA2, Human
The NCOA2 gene encodes nuclear receptor coactivator 2 which aids in the function of nuclear hormone receptors. Nuclear hormone receptors are conditional transcription factors that play important roles in various aspects of cell growth development and homeostasis by controlling expression of specific genes. Members of the nuclear hormone receptor superfamily which includes the 5 steroid receptors and class II nuclear receptors (see below) are structurally characterized by 3 distinct domains: an N-terminal transcriptional activation domain a central DNA-binding domain and a C-terminal hormone-binding domain. Before the binding of hormone steroid receptors which are sometimes called class I of the nuclear hormone receptor family remain inactive in a complex with heat-shock protein-90 (MIM 140571) and other stress family proteins. Binding of hormone induces critical conformational changes in steroid receptors that cause them to dissociate from the inhibitory complex bind as homodimers to specific DNA enhancer elements associated with target genes and modulate that gene's transcription. After binding to enhancer elements transcription factors require transcriptional coactivator proteins to mediate their stimulation of transcription initiation (Hong et al. 1997 [PubMed 9111344]).[supplied by OMIM Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.